Calculate the Rf values for each pigment and record the values in Data Table 1 (column E) using the following formula Rf = distance pigment travels distance the solvent front travels
- Rf for carotenes = 9.7cm/9.8cm = 0.99.
- Rf for xanthophylls = 7.2cm/9.8cm = 0.73.
- Rf for chlorophyll a = 5.1cm/9.8cm = 0.52.
- Rf for chlorophyll b = 3.7cm/9.8cm = 0.38.
What is the Rf value of each pigment line?
You can obtain Rf values for each of your pigment lines using the equation in Figure 1. The literature reports the following Rf values for each component: Rf = 0.16 for xanthophyll, Rf = 0.32 for chlorophyll b, Rf = 0.44 for chlorophyll a, and Rf = 0.95 for β- carotene. Furthermore, which pigment has the highest RF value?
How do you determine the Rf value of a compound?
The farther a compound travelled, the larger it's Rf value. Logically, you can conclude that if a compound A travels farther than compound B in a polar solvent, then it is more polar than solvent B. Also, how is an RF value useful?
What is the Rf value in this experiment?
Rf Value = Distance from Baseline travelled by Solute Distance from Baseline travelled by Solvent (Solvent Front)
What is the formula for chromatography RF?
In other words, Rf = (distance moved by solute) / (distance moved by solvent). In chromatography, a mixture of pigments to be measured is applied close to the bottom of a strip of chromatography paper. This strip of paper is then placed in solvent, so that only the bottom edge of the paper touches the solvent.
How is the Rf for each pigment in chromatography calculated?
5:266:15Chromatography Instructions and RF Value Calculations - YouTubeYouTubeStart of suggested clipEnd of suggested clipTake the distance the pigment traveled such as chlorophyll b which is one point eight eighteenMoreTake the distance the pigment traveled such as chlorophyll b which is one point eight eighteen millimeters. And then divide it by the distance the solvent traveled which is how high the solvent.
What is the Rf value for pigment chromatography?
4:115:322.9 Separation of Photosynthetic Pigments by Chromatography ...YouTubeStart of suggested clipEnd of suggested clipIn order to identify the pigment. You first need to calculate the RF value for each pigment that youMoreIn order to identify the pigment. You first need to calculate the RF value for each pigment that you found on your chromatogram. To do this for each one you want to take the distance. Move by the
Why do pigments have different Rf values?
The pigments are carried at different rates because they are not equally soluble. A pigment that is the most soluble will travel the greatest distance and a pigment that is less soluble will move a shorter distance.
How do you tell which pigment is most soluble?
The orange colored band, made of the pigment called carotenoids. is the most soluble in alcohol, so it traveled the farthest. The yellow xanthophylls are the next most soluble, followed by the blue-green chlorophyll A. The least soluble pigment is the yellow green chlorophyll B.
What is the Rf value of a pigment?
Rf value is a ratio of the distance travelled on chromatography paper by a pigment ...
Where is the mixture of pigments applied in chromatography?
In chromatography, a mixture of pigments to be measured is applied close to the bottom of a strip of chromatography paper. This strip of paper is then placed in solvent, so that only the bottom edge of the paper touches the solvent.
Why do pigments move up in chromatography?
Because of the paper's capillary action, the solvent move s up the chromatography paper, and as it crosses the area where the pigments were applied, the pigments dissolve and move up the paper. The pigments that are more soluble move more quickly up the paper, and the less soluble pigments lag behind. After the pigments have stopped migrating, the ...
What is the Rf value of a solvent?
Due the fact that the solvent front is always larger from the distance travelled by the solute, Rf values are always between 0 - one extreme where solute remains fixed at its origin and 1 - the other extreme where the solute is so soluble that it moves as far as the solvent.
Does a compound travel the same distance along the stationary phase?
A particular compound will travel the same distance along the stationary phase by a specific solvent (or solvent mixture) given that other experimental conditions are kept constant. In other words, every compound (dye, pigment, organic substance etc) have a specific Rf value for every specific solvent and solvent concentration. ...
