Rothera’s Test for Ketone Bodies: Principle, Procedure and Clinical Significances
- Principle of Rothera’s Test. Acetoacetic acid and acetone react with alkaline solution of sodium nitroprusside to form a purple colored complex.
- Requirements. Mix and pulverize.
- Procedure of Rothera’s Test. Transfer about 5 ml of urine to a test tube. ...
- Observations and Results. ...
What is Rothera’s test?
Rothera’s test Principle, Procedure, Result. Acetoacetic acid and acetone react with an alkaline solution of sodium nitroprusside to form a purple-colored complex. This method can detect above...
What is Rothera's test by nitroprusside reaction?
Rothera’s test by Nitroprusside reaction: Rothera’s test by Nitroprusside reaction is a specific test used for the determination of ketone bodies. The following ketones bodies or acetone bodies are found in the urine during a condition called “ketosis.”
What is Rothera's test for acetone?
Also to know is, what is Rothera's test? method of detecting acetone and acetoacetic acid in urine. It is performed by saturating the sample with solid ~mmonium sulphate, adding a few drops of dilute sodium nitroprusside solution, and then adding excess of concentrated ammonia.
What chemicals are used in Rothera test?
Rothera’s test chemicals required: Powered ammonium sulphate. 5% sodium nitroprusside. Concentrated aqueous ammonia. Rothera’s test procedure: Take 5 ml of urine in a test tube. Saturate it with powdered ammonium sulphate. Add 1 ml of 5% sodium nitroprusside. Mix thoroughly.
What is Rothera's test?
ROTHERA'S test(l) is well known in humah and veterinary medicine as a. method of detecting acetone and acetoacetic acid in urine.
What is the principle of Acetest?
A buffer provides the optimum pH for the reaction. 2.2 Acetest will detect as little as 5 mg of acetoacetic acid/dL in urine. Acetest is specific for the detection of acetone and acetoacetic acid. It is about 10 times more sensitive to acetoacetic acid than acetone and will not react with betahydroxybutyric acid.
What is the principle of Gerhardt's test?
Principle of Gerhardt's test: The reagent is 10% ferric chloride. It is based on the reaction of ferric chloride (FeCl3) with acetoacetic acid only, and it will form red color.
What is the principle of ketone?
Ketone bodies: acetoacetate (AcAc) is the principle ketone body. It is produced and utilized during intermediary metabolism and other ketone bodies are derived from it.
What is the principle involved in most chemical examinations for urine protein?
The principle of ascorbic acid detection utilises phosphomolybdenic acid, which is reduced with ascorbic acid to molybdenum blue. The test is not specific for ascorbic acid as other strong reducing agents would react in a similar manner.
What is the principle of the protein error of indicators reaction?
Testing for protein in the urine is based on the phenomenon called the "protein error of indicators" (ability of protein to alter the color of some acid-base indicators without altering the pH). In a solution that does not contain protein, tetrabromphenol blue, buffered at a pH of 3, is yellow.
What is Hays Sulphur test?
Hay's test, also known as Hay's sulphur powder test, is a chemical test used for detecting the presence of bile salts in urine.
What is beta hydroxybutyrate test?
The Stanbio Chemistry LiquiColor® Beta-Hydroxybutyrate (B-HB) reagent is used to detect ketones to identify patients suffering from diabetic ketoacidosis, amongst many other clinical applications.
What test is used to detect ketones in urine?
Urine ketones are usually measured as a "spot test." This is available in a test kit that you can buy at a drug store. The kit contains dipsticks coated with chemicals that react with ketone bodies. A dipstick is dipped in the urine sample. A color change indicates the presence of ketones.
What is the difference between beta oxidation and ketogenesis?
1:4810:50Beta Oxidation and Ketone Bodies - NBDE Biochemistry - YouTubeYouTubeStart of suggested clipEnd of suggested clipSo carnitine acyltransferase one regulatory enzyme of beta oxidation. It's inhibited by malone AlcoaMoreSo carnitine acyltransferase one regulatory enzyme of beta oxidation. It's inhibited by malone Alcoa. And it's what transfers the fatty acid. Into the liver mitochondria.
What are ketones used for?
The ketones, a fatty acid, are then released from the liver and go into your bloodstream and are used as fuel to drive the body's metabolism and to support muscle function. The body typically needs ketones when insulin levels are low.
What is the purpose of ketogenesis?
At the same time, ketogenesis, i.e. the production by the liver of the ketone bodies β-hydroxybutyrate and acetoacetate (AcAc), is a physiologically important process to produce an alternative metabolic source of energy during the neonatal period, starvation or prolonged physical effort [30].
Rothera's Test for Ketone Bodies in Urine
Rothera’s test is a sort of laboratory test that is used to detect ketone bodies in urine qualitatively.
Objectives
The main aim of Rothera’s test is to detect the presence of Ketone bodies within the supplied urine sample.
Principle
An alkaline solution of sodium nitroprusside reacts with acetoacetic acid and acetone to generate a purple-colored complex. This method can detect acetoacetic acid concentrations of 1-5 mg/dl and acetone concentrations of 10-20 mg/dl. There is no beta-hydroxybutyrate found.
Procedure
Take a clean test tube and add 5 ml of urine to it. Transfers 1 gm of Rothera’s powder mixture within the test tube and mix well.
Inference
Ketone (acetone) bodies include acetone, acetoacetic acid and β-hydroxybutyric acid. To detect the latter a modified test has to be done. (oxidize the β-hydroxybutyric acid with hydrogen peroxide to form acetoacetic acid. Add a few drops of acetic acid to 2 ml of 1:1 diluted urine with distilled water.
Results
Positive Test: Ketone bodies are present if a purple permanganate-colored ring forms quickly at the contact.
