what is cmf pbs

by Tania Bergstrom Published 3 years ago Updated 3 years ago

phosphate-buffered saline solution (CMF-PBS) or serum-free medium. 2. Resuspend the cells in a solution of a proteolytic enzyme. A 0.25% solution of trypsin in CMF-PBS is most often used. Other enzymes.

What is CMF used to treat?

CMF can be used to treat primary breast cancer – breast cancer that has not spread beyond the breast or the lymph nodes (glands) under the arm. Chemotherapy is given to reduce the risk of breast cancer returning or spreading.

What is a cycle of CMF?

The treatment and period of time before the next one starts is called a cycle. CMF is normally given every three or four weeks. You may have four or six cycles of treatment. The total length of treatment is usually four to six months. 6. Side effects of CMF Like any treatment, CMF can cause side effects.

What is the difference between chemotherapy and CMF?

Chemotherapy is given to reduce the risk of breast cancer returning or spreading. CMF is usually given a few weeks after surgery, known as adjuvant (additional) therapy.

How long do you have to take CMF?

CMF is normally given every three or four weeks. You may have four or six cycles of treatment. The total length of treatment is usually four to six months. 6. Side effects of CMF Like any treatment, CMF can cause side effects. Everyone reacts differently to drugs and some people have more side effects than others.

Is PBS the same as saline?

Saline will have a pH similar to the water supply used while PBS is usually buffered with phosphates to pH7. 2. And while PBS can maintain this pH saline can not and cells like to be in a pH range 6.8-7.6 for optimal vitality.

What is difference between PBS Dpbs?

PBS is a commonly used buffer with a simple formulation, while DPBS also includes potassium chloride and is available in a larger variety of formulations, including with or without calcium and magnesium and with or without glucose and pyruvate.

What is PBS solution used for?

PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

What is PBS in microbiology?

Phosphate buffered saline (PBS) is a non-toxic solution used in many biological laboratories. Unlike water, PBS prevents cells rupturing or shrivelling up due to osmosis.

Are DPBS and PBS interchangeable?

The substances can often be used interchangeably, although dPBS is typically slightly lower in phosphate concentration and may include calcium and/or magnesium. Experimental needs will dictate which solution should be employed.

What is Dulbecco's phosphate buffered saline?

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

What is PBS used for in DNA extraction?

PBS is a balanced salt solution that maintains pH, osmotic balance and is therefore frequently used as a wash buffer in cell and tissue culture. PBS storage has been recommended by manufacturers protocols and has been previously used when examining various extraction kits12,30.

What does PBS do in cell culture?

Maintaining balance in your cell cultures PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, including washing cells before dissociation, transporting cells, diluting cells for counting, and preparing reagents.

What is phosphate buffered saline solution?

Phosphate-buffered saline (abbreviated PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate.

How does PBS adjust pH?

Add 0.245 g of Potassium Phosphate Monobasic to the solution. Adjust solution to desired pH (typically pH ≈ 7.4). Add distilled water until the volume is 1 L....PBS (Phosphate Buffered Saline) (1X, pH 7.4) Preparation and Recipe.ComponentAmountConcentrationPotassium Phosphate Monobasic (mw: 136.09 g/mol)0.245 g0.0018 M3 more rows

How do you calculate PBS?

Preparing PBS 1X by Volume Phosphate-buffered saline (PBS) is an isotonic solution that is used in many biological research applications. To make 1 L of PBS, add 100 mL of 10X PBS to 900 mL of water. This PBS recipe contains 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, and 1.8 mM KH2PO4.

What is 10X PBS?

10X PBS (Phosphate Buffered Saline) is a 10 fold concentrated, sterile, pH adjusted blend of phosphate buffer and saline solution, which when diluted 1 to 10 in ultrapure water produces a phosphate buffered saline of 8mM NaH2PO4, 150mM NaCl, 3mM KCl, and 2mM KH2PO4 at pH 7.4.

What is the difference between PBS and TBS?

PBS is Phosphate Buffered Saline which is PH 7.4 and usually used in cell culture, TBS is Tris-Hcl Buffered Saline which is PH7.

Why is PBS without calcium and magnesium?

DPBS without Calcium and Magnesium is used in the dissociation process to wash and resuspend cells when the presence of Calcium and Magnesium may inhibit Trypsin activity.

How much salt is Dpbs?

Catalog Number: 02-023-1AComponentsMole. WeightConcentration (g/L)Potassium Chloride (KCl)750.20Potassium Phosphate Monobasic (KH2PO4)1360.20Sodium Chloride (NaCl)58.448.00Sodium Phosphate Dibasic (Na2HPO4)1421.15

What is the pH of Dpbs?

from 7.2-7.6Dulbecco's Phosphate Buffered Saline, shortened to DPBS, is water-salt solution used to maintain a consistent pH in biological and chemical applications. It contains disodium hydrogen phosphate and sodium chloride. The pH of standard and custom PBS formulations ranges from 7.2-7.6 when the buffer is ready to use.

2. How does CMF work?

CMF chemotherapy destroys cancer cells by interfering with their ability to divide and grow.

3. When might CMF be prescribed?

4. How is CMF given?

CMF is given as a drip into a vein (intravenously) in the hand or arm, although there are other ways of giving it depending on factors such as how easy it is for chemotherapy staff to find suitable veins, and your preferences.

5. How long does treatment take?

You’ll normally be given your treatment at hospital as an outpatient or day case, which means you’ll be able to go home on the same day. Although it only takes about an hour, you’re likely to be at the hospital for longer than this.

6. Side effects of CMF

Like any treatment, CMF can cause side effects. Everyone reacts differently to drugs and some people have more side effects than others. These can usually be controlled and those described here will not affect everyone.

7. Blood clots

People with breast cancer have a higher risk of blood clots. Their risk is higher because of the cancer itself and some treatments for breast cancer. Having CMF increases the risk of blood clots such as deep vein thrombosis (DVT). People with a DVT are at risk of developing a pulmonary embolism.

8. Sex, contraception and pregnancy

You can still have sex during treatment. It’s not known if chemotherapy drugs can pass into vaginal fluids (or semen). Most hospital specialists advise using barrier methods of contraception, such as condoms, for a few days after chemotherapy is given.

Most recent answer

If you use DPBS and PBS for keeping your cells in incubator, your cells under go osmotic pressure and die. So according to my view it is not a good idea.

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