What does it mean if your blood is lipemic?
Feb 09, 2020 · Lipemia is presence of a high concentration of lipids (or fats) in the blood. When donated blood is lipemic it causes the plasma-containing products to …
Is lipemic blood dangerous?
(li-pe'me-a) [ lipo- + -emia] An abnormal amount of fat in the blood. alimentary lipemia An accumulation of fat in the blood after eating. lipemia retinalis A condition in which retinal …
How to pronounce lipemic?
Lipemia or serum lipemic is the result of high concentrations of lipids in the blood. This causes turbidity or opacity of the blood serum due to the suspension of fatty particles in it; however, not all lipids produce turbidity of the serum. Lipemia is caused by the presence of chylomicrons and very low-density lipoproteins (VLDL).
What does "slightly lipemic" mean?
Feb 15, 2014 · Lipemia is a turbidity of the sample caused by accumulation of lipoprotein particles. As lipoproteins vary in sizes, not all classes contribute equally to the turbidity. The largest particles, chylomicrons, with sample size of 70–1000 nm, have the greatest potential in causing turbidity of the sample.
What causes a blood sample to be lipemic?
The most common cause of lipemia is that the patient is not fasting and has eaten close in time to the blood draw. This effect is most dramatic when the patient has consumed a meal with high fat content. However, nonfasting on its own usually does not result in enough lipemia to significantly impact laboratory tests.Nov 22, 2019
How do you get rid of lipemia?
Centrifugation. A recommended procedure for treating lipemic samples is centrifugation using ultracentrifuge which effectively removes lipids and allows measurement of large number of analytes (42,43). However, due to the high cost, this equipment it is not available in a large number of laboratories.
What about lipemic blood What effect does this sample have on results?
Conclusion: Lipemia causes clinically significant interferences for phosphorus, creatinine, total protein and calcium measurement and those interferences could be effectively removed by ultracentrifugation.
What does lipemia affect?
Conclusion: Lipemia causes clinically significant interferences for phosphorus, creatinine, total protein and calcium measurement and those interferences could be effectively removed by ultracentrifugation.
What does high lipemia index mean?
Conclusion: A markedly elevated lipemia index in a clear serum sample measured on Siemens analyzers Dimension indicates a high possibility for the presence of a paraprotein in the sample.
What do chylomicrons transport?
Chylomicrons. Chylomicrons (Fig. 20-14) are formed in the intestinal epithelium to transport long-chain triglycerides to the tissues. Medium- and short-chain fats are transported directly to the liver through the portal circulation without packaging into lipoprotein particles.
What is the clinical significance of turbid or lipemic serum?
Turbidity can falsely elevate a hemoglobin level and thus result in miscalculating RBC indices (MCH and MCHC). It has also been reported that lipemic specimens analyzed on optical hematology instruments may also erroneously impact white blood cells counts (WBC) and platelet counts.Feb 22, 2016
How does lipemia affect a laboratory?
This mechanism is probably the most common way in which lipemia affects results of laboratory tests. Lipoprotein particles in the sample can absorb light. The amount of absorbed light is inversely proportional to the wavelength and decreases from 300 to 700 nm, with no specific absorption peaks in between ( 22 ). Therefore, methods that use lower wavelengths are more affected by lipemia, because the absorbance is the highest in that part of the spectra. Many clinical chemistry methods (like alanine aminotranspherase, ALT; aspartate aminotranspherase, AST; glucose) use reaction NAD (P) + ↔ NAD (P)H + H + as an indicator reaction for determining concentration or activity of the analyte. Since the change of absorbance is measured at 340 nm, most of these methods are strongly affected by lipemia.
What is lipemia interference?
In the clinical laboratory setting, interferences can be a significant source of laboratory errors with potential to cause serious harm for the patient. After hemolysis, lipemia is the most frequent endogenous interference that can influence results of various laboratory methods by several mechanisms. The most common preanalytical cause of lipemic samples is inadequate time of blood sampling after the meal or parenteral administration of synthetic lipid emulsions. Although the best way of detecting the degree of lipemia is measuring lipemic index on analytical platforms, laboratory experts should be aware of its problems, like false positive results and lack of standardization between manufacturers. Unlike for other interferences, lipemia can be removed and measurement can be done in a clear sample. However, a protocol for removing lipids from the sample has to be chosen carefully, since it is dependent on the analytes that have to be determined. Investigation of lipemia interference is an obligation of manufacturers of laboratory reagents; however, several literature findings report lack of verification of the declared data. Moreover, the acceptance criteria currently used by the most manufacturers are not based on biological variation and need to be revised. Written procedures for detection of lipemia, removing lipemia interference and reporting results from lipemic samples should be available to laboratory staff in order to standardize the procedure, reduce errors and increase patient safety.
What is the turbidity of a sample?
Lipemia is a turbidity of the sample caused by accumulation of lipoprotein particles. As lipoproteins vary in sizes, not all classes contribute equally to the turbidity. The largest particles, chylomicrons, with sample size of 70–1000 nm, have the greatest potential in causing turbidity of the sample. Accumulation of small particles, high density lipoproteins (HDL), low density lipoproteins (LDL) and small very low density lipoproteins (VLDL) doesn’t result with lipemic samples ( Figure 1) ( 9 ).
Can lipemia be removed from a sample?
In most cases, lipemia can be removed from the sample and measurement can be done in a clear sample without interferences. There are several ways of removing lipids, and laboratory experts should carefully choose which one to use depending on the tests that have to be measured in the sample.
Can you measure lipid fractions?
For analytes distributed in the lipid layer, methods that remove lipid fraction are not acceptable. In such cases, measurement can be done in a diluted patient sample. Sample can be diluted only enough to remove the turbidity interference, but not too much to make sure that analyte concentration remains within the analytical limits of the tested methods (2 or 3 fold). This is probably the best approach for measurement of therapeutic drugs in lipemic samples.
Does triglyceride concentration correlate with lipemia?
The proportion of triglycerides differs among lipoprotein subclasses and ranges between approximately 50% in VLDL particles up to 85–90% in chylomicrons. Thus, the degree of turbidity does not correlate well with the triglyceride concentration. Twomey et al. confirmed this in an experiment where they demonstrated a linear agreement between triglyceride concentration and lipemic index when lipemia was simulated by an addition of standardized lipemic emulsion (Ivelip) (r 2 for Deming regression = 0.9994) ( 35 ). However, when lipemic index was correlated with triglyceride concentrations in patient sample, for both, visually turbid and non-turbid sample, the agreement was much lower (r 2 = 0.2399 and 0.7795, respectively).
