What does Benedict's solution test for?
The Benedict's test identifies reducing sugars (monosaccharide's and some disaccharides), which have free ketone or aldehyde functional groups. Benedict's solution can be used to test for the presence of glucose in urine. Click to see full answer. Keeping this in view, what color does Benedict's solution turn when positive?
What is Benedict's reagent?
What is Benedict’s reagent? Benedict’s reagent, also known as Benedict’s solution, is a chemical reagent which is made up of a complex mixture of sodium citrate, sodium carbonate, and the pentahydrate of copper (II) sulfate. When exposed to reducing sugars and other reducing substances, Benedict’s reagent changes its colour from clear blue ...
What is the composition of Benedict's solution?
Unsourced material may be challenged and removed. Benedict's reagent (often called Benedict's qualitative solution or Benedict's solution) is a chemical reagent and complex mixture of sodium carbonate, sodium citrate, and copper (II) sulfate pentahydrate.
How do you use Benedict’s solution in a urine test?
A mixture containing the reducing sugar (~ 8 drops of urine) and Benedict’s solution (~5 ml) are heated in a test tube for two minutes and allow it to cool. The color of the mixture will change accordingly resulting in precipitates, which indicates positive results. The results of the test can be summarized in the table below [6 – 8].
What does Benedict's reagent detect?
In lab, we used Benedict's reagent to test for one particular reducing sugar: glucose. Benedict's reagent starts out aqua-blue. As it is heated in the presence of reducing sugars, it turns yellow to orange. The "hotter" the final color of the reagent, the higher the concentration of reducing sugar.
Which sugar gives positive Benedict's test?
The reducing sugars that show positive results with benedict's solution are glucose, fructose, maltose etc. The correct option is D i.e. sucrose. Additional Information: Sucrose is a disaccharide composed of two monosaccharide molecules that are glucose and fructose.
How does Benedict's solution detect sugar?
The principle of Benedict's test is that when reducing sugars are heated in the presence of an alkali they get converted to powerful reducing species known as enediols. When Benedict's reagent solution and reducing sugars are heated together, the solution changes its colour to orange-red/ brick red.
Which sugar does not give Benedict's test?
SucroseSucrose is thus a non-reducing sugar, which does not react with Benedict's reagent.
What does Benedict’s test detect?
The primary application of Benedict’s test is to detect the presence of simple carbohydrates in an unidentified analyte. This test can be used to c...
What is Benedict’s reagent?
Benedict’s reagent, also known as Benedict’s solution, is a chemical reagent which is made up of a complex mixture of sodium citrate, sodium carbon...
How can Benedict’s test be performed?
Benedict’s test can be performed by taking one millilitre of the analyte solution in a test tube and mixing it with two millilitres of Benedict’s r...
How is Benedict’s solution prepared?
Benedict’s solution (or Benedict’s reagent) can be prepared by complexing cupric ions (Cu2+ cations) from the copper sulfate pentahydrate with citr...
List some substances that give positive results for Benedict’s test.
Some examples of substances that yield positive results for Benedict’s test are listed below. Glucose Fructose Ribose
1. What is the chemical formula of Benedict’s Solution?
This solution is prepared by mixing copper sulphate pentahydrate, sodium citrate, and sodium carbonate in distilled water. The result of the soluti...
2. Do complex carbohydrates give you a positive result in Benedict's solution?
It can be broken down in the process of digestion too. The sugar needs to be broken down into glucose and fructose then only the glucose test will...
3. How is Benedict’s Test and Barfoed’s Test Different?
Benedict’s test is used for determining the presence of reducing sugar. On the other hand, Barfoed’s test is used for finding if the sugar is a mon...
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