RNA annotation and mapping of promoters for analysis of gene expression (RAMPAGE) is a method that harnesses highly specific sequencing of 5′-complete complementary DNAs to identify transcription start sites (TSSs) genome-wide.
What is Rampage?
Learn more. RNA annotation and mapping of promoters for analysis of gene expression (RAMPAGE) is a method that harnesses highly specific sequencing of 5′-complete complementary DNAs to identify transcription start sites (TSSs) genome-wide.
What are rampage assays used for?
The ENCODE-developed pipeline for RAMPAGE assays is also used for the analysis of CAGE (Cap Analysis Gene Expression), and can process libraries generated using rRNA-depleted total RNA >200 nucleotides in size.
What is the difference between the cage and Rampage methods?
The CAGE method is intended to provide information on the 5' end of mRNA, and by extensions, TSSs; RAMPAGE is an improvement of the CAGE method 1. Reads must meet the criteria outlined under the Uniform Processing Pipeline Restrictions.
What is the tone of the movie Rampage?
“Rampage” opens by establishing its ridiculous tone early. A scientist on a space station is struggling to save some genetically engineered samples from, well, a mutated super-rat. The station is on fire, and everyone else appears dead, but she’s ordered to save the science.
What is a RAMPAGE?
RAMPAGE (RNA Annotation and Mapping of Promoters for the Analysis of Gene Expression) is a sequencing approach designed to identify transcription start sites (TSSs) at base-pair resolution, quantify their expression, and characterize their transcripts. The assay uses direct cDNA evidence to link specific genes and their regulatory TSSs 1.
What is RAMPAGE pipeline?
The RAMPAGE pipeline was developed as a part of the ENCODE Uniform Processing Pipelines series. The full RAMPAGE pipeline code is freely available on Github and can be run on DNAnexus (link requires account creation) at their current pricing.
What is the movie Rampage based on?
Dwayne Johnson finally gets a star to match his size if not charisma in the sometimes gloriously ludicrous “Rampage,” a movie based on a hit arcade game that was literally just larger-than-life monsters bashing buildings. If you’re thinking, “Wow, that seems like a flimsy premise from which to adapt a feature film,” you’re not entirely wrong. Directed by “ San Andreas ” helmer Brad Peyton, this CGI blockbuster is often a defiantly stupid movie, most comfortable with what it’s trying to accomplish when giant monsters are destroying half of downtown Chicago or leaping on moving helicopters. It’s the narrative tissue in between that allows the movie magic to falter every now and then. However, when Johnson is doing that movie action star thing he does so well and giant animals are going enormous-mano-a-enormous-mano, there’s undeniably goofy fun to be had. You just have to be patient during the downtime.
Is there a rat in the movie Rampage?
Yes, “Rampage” opens with a super space rat aboard an on-fire spaceship hurtling to Earth. It’s certainly a tone setter. The samples plummet to Earth, and land in three locations. One happens to hit ground in a San Diego Wildlife Sanctuary managed by Davis Okoye (Johnson).