Receiving Helpdesk

quaternary protein structure

by Prof. Adrian Ernser Published 3 years ago Updated 3 years ago

Protein quaternary structure refers to the structure of proteins which are themselves composed of two or more smaller protein chains (also referred to as subunits). Protein quaternary structure describes the number and arrangement of multiple folded protein subunits in a multi-subunit complex.

How is a quaternary protein structure formed?

Quaternary structure is the interaction of two or more folded polypeptides. Many proteins require the assembly of several polypeptide subunits before they become active. If the final protein is made of two subunits, the protein is said to be a dimer.

What is the function of a quaternary protein structure?

Quaternary structure is an important protein attribute that is closely related to its function. Proteins with quaternary structure are called oligomeric proteins. Oligomeric proteins are involved in various biological processes, such as metabolism, signal transduction, and chromosome replication.

Where are quaternary protein structures formed?

Quaternary Structure exists when more than one amino acid chain comes together to form a protein complex.

What are the advantages of quaternary structure?

There are many advantages a protein benefits from through its quaternary structure. A cell may conserve valuable resources in the creation of a large protein by repeating the synthesis of a few polypeptide chains many times rather than synthesizing one extremely long polypeptide chain.

What is the difference between tertiary and quaternary structure?

2 Tertiary and Quaternary Structures. Tertiary structure refers to the configuration of a protein subunit in three-dimensional space, while quaternary structure refers to the relationships of the four subunits of hemoglobin to each other.

What is an example of a quaternary structure?

The quaternary structure refers to the number and arrangement of the protein subunits with respect to one another. Examples of proteins with quaternary structure include hemoglobin, DNA polymerase, ribosomes, antibodies, and ion channels.

What bonds stabilize quaternary structure?

The quaternary structure is also stabilized by the non-covalent interactions and disulfide bonds as in the tertiary structure, where more than one polypeptide is held together to form a single functional unit called multimer.

What is the quaternary structure of a protein quizlet?

The Quaternary structure of the protein is two or more polypeptide chains bonded together, and maintained by the same interactions as the tertiary structure.

What is protein structure and function?

Proteins are built as chains of amino acids, which then fold into unique three-dimensional shapes. Bonding within protein molecules helps stabilize their structure, and the final folded forms of proteins are well-adapted for their functions.

Which of the following statements is true about the quaternary structure of a protein?

Question: Which of the following statements about quaternary structure is true? (d) Quaternary structure requires covalent interactions between polypeptide chains.

What must be true for a protein to have quaternary structure?

The joining of these independent subunits results in quaternary structure. In order for a protein to have quaterary structure, it must have multiple subunits; this means it must consists of at least two polypeptide chains.

How to determine quaternary structure of protein?

Protein quaternary structure can be determined using a variety of experimental techniques that require a sample of protein in a variety of experimental conditions. The experiments often provide an estimate of the mass of the native protein and, together with knowledge of the masses and/or stoichiometry of the subunits, allow the quaternary structure to be predicted with a given accuracy. It is not always possible to obtain a precise determination of the subunit composition for a variety of reasons.

What is quaternary structure?

The quaternary structure refers to the number and arrangement of the protein subunits with respect to one another. Examples of proteins with quaternary structure include hemoglobin, DNA polymerase, and ion channels . Enzymes composed of subunits with diverse functions are sometimes called holoenzymes, in which some parts may be known as regulatory ...

What is the term for a protein that is made from multiple copies of a polypeptide?

When multiple copies of a polypeptide encoded by a gene form a quaternary complex, this protein structure is referred to as a multimer . When a multimer is formed from polypeptides produced by two different mutant alleles of a particular gene, the mixed multimer may exhibit greater functional activity than the unmixed multimers formed by each of the mutants alone. In such a case, the phenomenon is referred to as intragenic complementation (also called inter-allelic complementation). Intragenic complementation appears to be common and has been studied in many different genes in a variety of organisms including the fungi Neurospora crassa, Saccharomyces cerevisiae and Schizosaccharomyces pombe; the bacterium Salmonella typhimurium; the virus bacteriophage T4, an RNA virus, and humans. The intermolecular forces likely responsible for self-recognition and multimer formation were discussed by Jehle.

How many subunits are in an oligomeric complex?

Formal and Greco-Latinate names are generally used for the first ten types and can be used for up to twenty subunits, whereas higher order complexes are usually described by the number of subunits, followed by -meric.

How to determine the number of subunits in a protein complex?

The number of subunits in a protein complex can often be determined by measuring the hydrodynamic molecular volume or mass of the intact complex , which requires native solution conditions.

Which organisms have intragenic complementation?

Intragenic complementation appears to be common and has been studied in many different genes in a variety of organisms including the fungi Neurospora crassa, Saccharomyces cerevisiae and Schizosaccharomyces pombe; the bacterium Salmonella typhimurium; the virus bacteriophage T4, an RNA virus, and humans.

What is dual polarisation interferometry?

Dual polarisation interferometry (meas ures the size and the density of the complex)

What is quaternary structure?

Quaternary structure refers to the further stabilization of the protein molecule by bonding with one or more similar tertiary structures via further non-covalent interactions and disulfide bonding.

How to determine quaternary structure?

The quaternary structure is usually determined by X-ray crystallography, as described previously. However, when crystallographic data were difficult or impossible to gather, electron microscopy had provided some clues to quaternary structure.

What is the structure of deoxyhemoglobin?

Structure of Deoxyhemoglobin. The α and β subunits of hemoglobin interact cooperatively, and when one heme binds O 2, then each of the others rapidly binds O 2. The subunits of hemoglobin do not act independently. When one subunit binds O 2, its conformation changes.

What are the subunits of proteasomes?

Both types of subunits, α and β , are members of the N-terminal nucleophile (Ntn) hydrolase superfamily. The proteasome subunits are well conserved: the different subunits have at least 50% similarity, while there is only ~30% similarity between the seven subunits within the different subunit types in same species, and even less among the α - and β -subunits. Ntn hydrolases consist of two central antiparallel β -sheets, which are flanked on either side by α -helices and open at one end to form the active-site cleft ( Figures 1 (d) and 1 (e) ). The α -subunits possess an N-terminal extension of the Ntn core structure, filling the active-site cleft, which renders α -subunits proteolytically inactive ( Figure 1 (d) ).

What was the first protein to be determined by crystallography?

Hemoglobin (Hb) was more than just the first protein whose structure was determined by crystallography—it was the first oligomeric protein. So, in addition to the information that described the conformation of the α and β chains, the arrangement of subunits in a single Hb tetramer was also determined. From these early results and those of other researchers working on spherical viruses, a set of rules governing the assembly of subunits into limited aggregates evolved. The rules center around symmetry arguments, and make it possible to predict, or at least set some usable principles to describe, the quaternary structure of any one oligomeric protein.

Which subunits of hemoglobin interact cooperatively?

The α and β subunits of hemoglobin interact cooperatively, and when one heme binds O 2, then each of the others rapidly binds O 2.

How are subunits held together?

The subunits are held together by hydrogen bonds and van der Waals forces between nonpolar side chains. The subunits in a quaternary structure must be specifically arranged for the entire protein to function properly.

Why are protein structures quaternary?

The arrangement of protein structures can be described as quaternary due to the multilevel nature of these structures. Learn about the definition and functions of the quaternary protein structure, and see an overview of homomultimeric and heteromultimeric proteins. Updated: 10/10/2021

What is another name for a protein with quaternary structure?

Another protein subunit may be the catalytic subunit. Another name for a protein with quaternary structure is called a multiprotein complex .

Why do multimeric proteins undergo complex conformational changes?

This is due first to the fact that individual protein subunits within the complex can shift conformation. This is due second to the fact that the individual protein sub units can shift position with respect to each other. Learning Outcomes.

What is a multimeric protein called?

Multimeric proteins with different subunits are called heteromultimeric. When the subunits are all the same the multimeric protein is said to be homomultimeric. The majority of proteins in the Protein Data Bank (a library of 3-dimensional protein images submitted by biologists and biochemists) are homomultimeric.

How do protein subunits come together?

Protein subunits come together to form a protein. This image shows a protein that is made of several protein subunits. Each color represents a separate protein. Each protein subunit is produced individually by a ribosome. Then, each protein subunit attains secondary and tertiary structure. Finally, the protein subunits come together ...

What are homomultimeric proteins?

In fact, homomultimeric proteins are responsible for the diversity and specificity of many cellular pathways. They can also regulate gene expression, activity of enzymes, ion channels, receptors, and cell adhesion processes. Proteins have primary, secondary, tertiary, and quaternary structure.

What is quaternary structure?

By definition, quaternary structure is the arrangement of more than one protein molecule in a multi-subunit complex. The nomenclature here can get a bit confusing because we call a single polypeptide chain a protein if it can function on its own. However, many proteins are actually comprised of several polypeptide chains.

What is the Quaternary Structure of a Protein?

Proteins are complex molecules composed of multiple different combinations of twenty different amino acids. They fulfill many functions in the body, and examples of proteins include enzymes, antibodies, signaling and transport components, and more.

Do All Proteins Have Quaternary Structure?

All proteins contain an amino acid base sequence, and therefore, a primary structure. However, not all proteins contain all structural levels. Only those proteins that are made of multiple polypeptide chains have a quaternary structure, while proteins made from a single polypeptide do not.

Quaternary Structure Bonds

A protein with a quaternary structure is composed of several complex subunits. These subunits are bonded together through non-covalent bonds, primarily via hydrogen bonds and Van der Waals forces, the same weak interactions that promote tertiary structure in proteins as well.

What is the quaternary structure of proteins?

Quaternary Structure: Protein Chains Combine to Make Protein Complexes. Secondary and tertiary structures are determined by a protein's sequence of amino acids, or primary structure. All proteins have primary, secondary and tertiary structure. Some proteins are made up of more than one amino acid chain, giving them a quaternary structure.

What is a quaternary protein?

Some proteins are made up of more than one amino acid chain, giving them a quaternary structure . These multi-chain proteins are held together with the same forces as the tertiary structure of individual protein chains (hydrophobic, hydrophillic, positive/negative and cysteine interactions).

What is the structure of proteins?

A Review of Protein Structure 1 Proteins are long chains of amino acids that fold into complex 3-dimensional shapes. 2 Proteins come in an almost endless array of shapes and sizes, each type acting like a specialized molecular machine that performs a specific microscopic task. 3 Primary Structure is the specific order of amino acids in a protein polypeptide chain. There are 20 different types of amino acids that can be incorporated into a protein chain, each with unique attributes (hydrophobic, hydrophillic, positive, negative, and cysteine). 4 Secondary Structures are the alpha helices and beta pleated sheets present in a folded protein's structure. 5 Tertiary Structure is the final shape of an entire amino acid chain. This shape is directly related to the function of the protein. 6 Quaternary Structure exists when more than one amino acid chain comes together to form a protein complex.

What are long chains of amino acids that fold into complex 3-dimensional shapes?

Proteins are long chains of amino acids that fold into complex 3-dimensional shapes.

What is the final shape of an amino acid chain?

Tertiary Structure is the final shape of an entire amino acid chain. This shape is directly related to the function of the protein. Quaternary Structure exists when more than one amino acid chain comes together to form a protein complex.

How many different types of amino acids are there in a protein chain?

There are 20 different types of amino acids that can be incorporated into a protein chain, each with unique attributes (hydrophobic, hydrophillic, positive, negative, and cysteine). Secondary Structures are the alpha helices and beta pleated sheets present in a folded protein's structure.

What are the four levels of protein structure?

To understand how a protein gets its final shape or conformation, we need to understand the four levels of protein structure: primary, secondary, tertiary, and quaternary.

Which amino acid clusters on the inside of a protein?

Also important to tertiary structure are hydrophobic interactions, in which amino acids with nonpolar, hydrophobic R groups cluster together on the inside of the protein, leaving hydrophilic amino acids on the outside to interact with surrounding water molecules.

How are the amino acids in insulin connected?

Image of insulin. Insulin consists of an A chain and a B chain. They are connected to one another by disulfide bonds (sulfur-sulfur bonds between cysteines). The A chain also contains an internal disulfide bond. The amino acids that make up each chain of insulin are represented as connected circles, each with the three-letter abbreviation of the amino acid's name.

Why do egg whites have a specific shape?

Egg whites contain large amounts of proteins called albumins, and the albumins normally have a specific 3D shape, thanks to bonds formed between different amino acids in the protein. Heating causes these bonds to break and exposes hydrophobic (water-hating) amino acids usually kept on the inside of the protein.

How many polypeptide chains are there in insulin?

For example, the hormone insulin has two polypeptide chains, A and B, shown in diagram below. (The insulin molecule shown here is cow insulin, although its structure is similar to that of human insulin.) Each chain has its own set of amino acids, assembled in a particular order.

Where do the R groups of amino acids stick outward?

The R groups of the amino acids stick outward from the α helix, where they are free to interact. In a β pleated sheet, two or more segments of a polypeptide chain line up next to each other, forming a sheet-like structure held together by hydrogen bonds.

What are the different order of proteins?

Orders of protein structure: primary, secondary, tertiary, and quaternary. Alpha helix and beta pleated sheet.

How to determine the structure of a protein?

How to Determine Protein Structure Type. The three-dimensional shape of a protein is determined by its primary structure. The order of amino acids establishes a protein's structure and specific function. The distinct instructions for the order of amino acids are designated by the genes in a cell.

What are the two types of secondary structures in proteins?

This structure resembles a coiled spring and is secured by hydrogen bonding in the polypeptide chain. The second type of secondary structure in proteins is the beta (β) pleated sheet.

What is the role of hydrogen bonding in protein structure?

Hydrogen bonding in the polypeptide chain and between amino acid "R" groups helps to stabilize protein structure by holding the protein in the shape established by the hydrophobic interactions.

How many different types of protein are there?

Four Protein Structure Types. The four levels of protein structure are distinguished from one another by the degree of complexity in the polypeptide chain. A single protein molecule may contain one or more of the protein structure types: primary, secondary, tertiary, and quaternary structure. 1.

What is the amino acid sequence?

All amino acids have the alpha carbon bonded to a hydrogen atom, carboxyl group, and an amino group. The "R" group varies among amino acids and determines the differences between these protein monomers. The amino acid sequence of a protein is determined by the information found in the cellular genetic code.

What type of bonding is used to hold proteins together?

Folding in proteins happens spontaneously. Chemical bonding between portions of the polypeptide chain aid in holding the protein together and giving it its shape. There are two general classes of protein molecules: globular proteins and fibrous proteins.

How are amino acids determined?

The amino acid sequence of a protein is determined by the information found in the cellular genetic code. The order of amino acids in a polypeptide chain is unique and specific to a particular protein. Altering a single amino acid causes a gene mutation, which most often results in a non-functioning protein. 2.

What is a quaternary protein?

When several polypeptide chains (AKA subunits) come together, they can form a structure known as a quaternary protein. One example of a quaternary protein structure is hemoglobin. Hemoglobin is made up of four polypeptide chains, and is specially adapted to bind oxygen in the blood. Haemoglobin is a quaternary protein.

What is the structure of proteins?

The structure of proteins is directly related to their function and may be primary, secondary, tertiary, or quaternary.

What are the bonds formed between amino acids?

These include hydrogen bonds, ionic bonds, and disulfide bonds.

What determines the final 3D structure of a protein?

The amino acid sequence of a polypeptide chain determines the final 3D structure of the protein. There are four levels of protein structure; the primary structure, the secondary structure, the tertiary structure, and the quaternary structure. Furthermore, there are two main classes of 3D protein structures; these are globular and fibrous proteins.

How are amino acids determined in a polypeptide chain?

The order of amino acids in the polypeptide chain is determined by the order of nucleotides (the DNA sequence) of the gene that encodes it. Even a tiny change in the amino acid sequence of the polypeptide chain can alter the overall structure and function of the protein.

What is a protein made of?

Many proteins are made of a single polypeptide chain and don’t become any more complex than their tertiary structure. However, some proteins are made up of multiple polypeptide chains. When several polypeptide chains (AKA subunits) come together, they can form a structure known as a quaternary protein.

What is the function of a protein?

The function of a protein depends heavily on its final structure. Tertiary and quaternary proteins are both functional proteins with a 3D structure. However, the type of structure can vary significantly between different proteins. There are two main classes of 3D protein structure: globular proteins and fibrous proteins.

Overview

Protein quaternary structure is the fourth (and highest) classification level of protein structure. Protein quaternary structure refers to the structure of proteins which are themselves composed of two or more smaller protein chains (also referred to as subunits). Protein quaternary structure describes the number and arrangement of multiple folded protein subunits in a multi-subunit complex. It in…

Description and examples

Many proteins are actually assemblies of multiple polypeptide chains. The quaternary structure refers to the number and arrangement of the protein subunits with respect to one another. Examples of proteins with quaternary structure include hemoglobin, DNA polymerase, ribosomes, antibodies, and ion channels.
Enzymes composed of subunits with diverse functions are sometimes called holoenzymes, in wh…

Nomenclature

The number of subunits in an oligomeric complex is described using names that end in -mer (Greek for "part, subunit"). Formal and Greco-Latinate names are generally used for the first ten types and can be used for up to twenty subunits, whereas higher order complexes are usually described by the number of subunits, followed by -meric.

Structure Determination

Protein quaternary structure can be determined using a variety of experimental techniques that require a sample of protein in a variety of experimental conditions. The experiments often provide an estimate of the mass of the native protein and, together with knowledge of the masses and/or stoichiometry of the subunits, allow the quaternary structure to be predicted with a given accuracy. It is not always possible to obtain a precise determination of the subunit composition …

Structure Prediction

Some bioinformatics methods have been developed for predicting the quaternary structural attributes of proteins based on their sequence information by using various modes of pseudo amino acid composition.
Protein folding prediction programs used to predict protein tertiary structure have also been expanding to better predict protein quaternary structure. One such development is AlphaFold-M…

Role in Cell Signaling

Protein quaternary structure also plays an important role in certain cell signaling pathways. The G-protein coupled receptor pathway involves a heterotrimeric protein known as a G-protein. G-proteins contain three distinct subunits known as the G-alpha, G-beta, and G-gamma subunits. When the G-protein is activated, it binds to the G-protein coupled receptor protein and the cell signaling pathway is initiated. Another example is the receptor tyrosine kinase (RTK) pathway, w…

Protein–protein interactions

Proteins are capable of forming very tight complexes. For example, ribonuclease inhibitor binds to ribonuclease A with a roughly 20 fM dissociation constant. Other proteins have evolved to bind specifically to unusual moieties on another protein, e.g., biotin groups (avidin), phosphorylated tyrosines (SH2 domains) or proline-rich segments (SH3 domains). Protein-protein interactions can be engineered to favor certain oligomerization states.

Intragenic complementation

When multiple copies of a polypeptide encoded by a gene form a quaternary complex, this protein structure is referred to as a multimer. When a multimer is formed from polypeptides produced by two different mutant alleles of a particular gene, the mixed multimer may exhibit greater functional activity than the unmixed multimers formed by each of the mutants alone. In such a case, the phenomenon is referred to as intragenic complementation (also called inter-allelic complementat…

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 1 2 3 4 5 6 7 8 9
8.3.21PHP Version2.23sRequest Duration2MBMemory UsageGET {post}Route
  • warninglog[16:06:47] LOG.warning: Creation of dynamic property Barryvdh\Debugbar\DataFormatter\QueryFormatter:...
  • warninglog[16:06:47] LOG.warning: Creation of dynamic property Barryvdh\Debugbar\DataFormatter\QueryFormatter:...
  • warninglog[16:06:47] LOG.warning: Callables of the form ["Swift_SmtpTransport", "Swift_Transport_EsmtpTranspor...
  • warninglog[16:06:47] LOG.warning: Creation of dynamic property Barryvdh\Debugbar\DataFormatter\SimpleFormatter...
  • warninglog[16:06:47] LOG.warning: Creation of dynamic property Barryvdh\Debugbar\DataFormatter\SimpleFormatter...
  • warninglog[16:06:47] LOG.warning: json_decode(): Passing null to parameter #1 ($json) of type string is deprec...
  • warninglog[16:06:47] LOG.warning: json_decode(): Passing null to parameter #1 ($json) of type string is deprec...
  • warninglog[16:06:47] LOG.warning: json_decode(): Passing null to parameter #1 ($json) of type string is deprec...
  • warninglog[16:06:47] LOG.warning: json_decode(): Passing null to parameter #1 ($json) of type string is deprec...
  • warninglog[16:06:47] LOG.warning: json_decode(): Passing null to parameter #1 ($json) of type string is deprec...
  • warninglog[16:06:47] LOG.warning: mt_rand(): Passing null to parameter #2 ($max) of type int is deprecated in ...
  • Booting (11.53ms)
  • Application (2.22s)
  • 1 x Application (99.47%)
    2.22s
    1 x Booting (0.52%)
    11.53ms
    7 templates were rendered
    • themes.DevBlog.content.post (resources/views/themes/DevBlog/content/post.blade.php)34blade
      Params
      0
      post
      1
      postContent
      2
      author
      3
      updated_at
      4
      bing_rich_snippet_text
      5
      bing_rich_snippet_link
      6
      bing_related_keywords
      7
      google_related_keywords
      8
      bing_news_title
      9
      bing_news_description
      10
      bing_videos
      11
      bing_images
      12
      bing_search_result_title
      13
      bing_search_result_description
      14
      bing_search_result_url
      15
      bing_paa_questions
      16
      bing_paa_answers
      17
      bing_slider_faq_questions
      18
      bing_slider_faq_answers
      19
      bing_pop_faq_questions
      20
      bing_pop_faq_answers
      21
      bing_tab_faq_questions
      22
      bing_tab_faq_answers
      23
      google_faq_questions
      24
      google_faq_answers
      25
      google_rich_snippet
      26
      google_search_result
      27
      indexedArray
      28
      total_images
      29
      total_videos
      30
      settings
      31
      url_current
      32
      menus
      33
      sidebar
    • themes.DevBlog.layouts.master (resources/views/themes/DevBlog/layouts/master.blade.php)41blade
      Params
      0
      __env
      1
      app
      2
      errors
      3
      post
      4
      postContent
      5
      author
      6
      updated_at
      7
      bing_rich_snippet_text
      8
      bing_rich_snippet_link
      9
      bing_related_keywords
      10
      google_related_keywords
      11
      bing_news_title
      12
      bing_news_description
      13
      bing_videos
      14
      bing_images
      15
      bing_search_result_title
      16
      bing_search_result_description
      17
      bing_search_result_url
      18
      bing_paa_questions
      19
      bing_paa_answers
      20
      bing_slider_faq_questions
      21
      bing_slider_faq_answers
      22
      bing_pop_faq_questions
      23
      bing_pop_faq_answers
      24
      bing_tab_faq_questions
      25
      bing_tab_faq_answers
      26
      google_faq_questions
      27
      google_faq_answers
      28
      google_rich_snippet
      29
      google_search_result
      30
      indexedArray
      31
      total_images
      32
      total_videos
      33
      settings
      34
      url_current
      35
      menus
      36
      sidebar
      37
      i
      38
      __currentLoopData
      39
      loop
      40
      item
    • themes.DevBlog.panels.head (resources/views/themes/DevBlog/panels/head.blade.php)41blade
      Params
      0
      __env
      1
      app
      2
      errors
      3
      post
      4
      postContent
      5
      author
      6
      updated_at
      7
      bing_rich_snippet_text
      8
      bing_rich_snippet_link
      9
      bing_related_keywords
      10
      google_related_keywords
      11
      bing_news_title
      12
      bing_news_description
      13
      bing_videos
      14
      bing_images
      15
      bing_search_result_title
      16
      bing_search_result_description
      17
      bing_search_result_url
      18
      bing_paa_questions
      19
      bing_paa_answers
      20
      bing_slider_faq_questions
      21
      bing_slider_faq_answers
      22
      bing_pop_faq_questions
      23
      bing_pop_faq_answers
      24
      bing_tab_faq_questions
      25
      bing_tab_faq_answers
      26
      google_faq_questions
      27
      google_faq_answers
      28
      google_rich_snippet
      29
      google_search_result
      30
      indexedArray
      31
      total_images
      32
      total_videos
      33
      settings
      34
      url_current
      35
      menus
      36
      sidebar
      37
      i
      38
      __currentLoopData
      39
      loop
      40
      item
    • themes.DevBlog.panels.header (resources/views/themes/DevBlog/panels/header.blade.php)41blade
      Params
      0
      __env
      1
      app
      2
      errors
      3
      post
      4
      postContent
      5
      author
      6
      updated_at
      7
      bing_rich_snippet_text
      8
      bing_rich_snippet_link
      9
      bing_related_keywords
      10
      google_related_keywords
      11
      bing_news_title
      12
      bing_news_description
      13
      bing_videos
      14
      bing_images
      15
      bing_search_result_title
      16
      bing_search_result_description
      17
      bing_search_result_url
      18
      bing_paa_questions
      19
      bing_paa_answers
      20
      bing_slider_faq_questions
      21
      bing_slider_faq_answers
      22
      bing_pop_faq_questions
      23
      bing_pop_faq_answers
      24
      bing_tab_faq_questions
      25
      bing_tab_faq_answers
      26
      google_faq_questions
      27
      google_faq_answers
      28
      google_rich_snippet
      29
      google_search_result
      30
      indexedArray
      31
      total_images
      32
      total_videos
      33
      settings
      34
      url_current
      35
      menus
      36
      sidebar
      37
      i
      38
      __currentLoopData
      39
      loop
      40
      item
    • themes.DevBlog.panels.navbar (resources/views/themes/DevBlog/panels/navbar.blade.php)41blade
      Params
      0
      __env
      1
      app
      2
      errors
      3
      post
      4
      postContent
      5
      author
      6
      updated_at
      7
      bing_rich_snippet_text
      8
      bing_rich_snippet_link
      9
      bing_related_keywords
      10
      google_related_keywords
      11
      bing_news_title
      12
      bing_news_description
      13
      bing_videos
      14
      bing_images
      15
      bing_search_result_title
      16
      bing_search_result_description
      17
      bing_search_result_url
      18
      bing_paa_questions
      19
      bing_paa_answers
      20
      bing_slider_faq_questions
      21
      bing_slider_faq_answers
      22
      bing_pop_faq_questions
      23
      bing_pop_faq_answers
      24
      bing_tab_faq_questions
      25
      bing_tab_faq_answers
      26
      google_faq_questions
      27
      google_faq_answers
      28
      google_rich_snippet
      29
      google_search_result
      30
      indexedArray
      31
      total_images
      32
      total_videos
      33
      settings
      34
      url_current
      35
      menus
      36
      sidebar
      37
      i
      38
      __currentLoopData
      39
      loop
      40
      item
    • themes.DevBlog.panels.footer (resources/views/themes/DevBlog/panels/footer.blade.php)41blade
      Params
      0
      __env
      1
      app
      2
      errors
      3
      post
      4
      postContent
      5
      author
      6
      updated_at
      7
      bing_rich_snippet_text
      8
      bing_rich_snippet_link
      9
      bing_related_keywords
      10
      google_related_keywords
      11
      bing_news_title
      12
      bing_news_description
      13
      bing_videos
      14
      bing_images
      15
      bing_search_result_title
      16
      bing_search_result_description
      17
      bing_search_result_url
      18
      bing_paa_questions
      19
      bing_paa_answers
      20
      bing_slider_faq_questions
      21
      bing_slider_faq_answers
      22
      bing_pop_faq_questions
      23
      bing_pop_faq_answers
      24
      bing_tab_faq_questions
      25
      bing_tab_faq_answers
      26
      google_faq_questions
      27
      google_faq_answers
      28
      google_rich_snippet
      29
      google_search_result
      30
      indexedArray
      31
      total_images
      32
      total_videos
      33
      settings
      34
      url_current
      35
      menus
      36
      sidebar
      37
      i
      38
      __currentLoopData
      39
      loop
      40
      item
    • themes.DevBlog.panels.scripts (resources/views/themes/DevBlog/panels/scripts.blade.php)41blade
      Params
      0
      __env
      1
      app
      2
      errors
      3
      post
      4
      postContent
      5
      author
      6
      updated_at
      7
      bing_rich_snippet_text
      8
      bing_rich_snippet_link
      9
      bing_related_keywords
      10
      google_related_keywords
      11
      bing_news_title
      12
      bing_news_description
      13
      bing_videos
      14
      bing_images
      15
      bing_search_result_title
      16
      bing_search_result_description
      17
      bing_search_result_url
      18
      bing_paa_questions
      19
      bing_paa_answers
      20
      bing_slider_faq_questions
      21
      bing_slider_faq_answers
      22
      bing_pop_faq_questions
      23
      bing_pop_faq_answers
      24
      bing_tab_faq_questions
      25
      bing_tab_faq_answers
      26
      google_faq_questions
      27
      google_faq_answers
      28
      google_rich_snippet
      29
      google_search_result
      30
      indexedArray
      31
      total_images
      32
      total_videos
      33
      settings
      34
      url_current
      35
      menus
      36
      sidebar
      37
      i
      38
      __currentLoopData
      39
      loop
      40
      item
    uri
    GET {post}
    middleware
    web, checkdate
    as
    post.show
    controller
    App\Http\Controllers\Frontend\json_data\PostController@show
    namespace
    where
    file
    app/Http/Controllers/Frontend/json_data/PostController.php:18-166
    7 statements were executed2.2s
    • select * from `posts` where `published_at` <= '2025-06-19 16:06:47' and `slug` = 'quaternary-protein-structure' and `posts`.`deleted_at` is null limit 1
      2.25ms/app/Providers/RouteServiceProvider.php:54receivinghelpdeskask
      Metadata
      Bindings
      • 0. 2025-06-19 16:06:47
      • 1. quaternary-protein-structure
      Backtrace
      • 15. /app/Providers/RouteServiceProvider.php:54
      • 18. /vendor/laravel/framework/src/Illuminate/Routing/Router.php:842
      • 19. Route binding:39
      • 20. /vendor/laravel/framework/src/Illuminate/Pipeline/Pipeline.php:167
      • 21. /vendor/laravel/framework/src/Illuminate/Foundation/Http/Middleware/VerifyCsrfToken.php:78
    • select * from `json_post_contents` where `json_post_contents`.`post_id` = 83001 and `json_post_contents`.`post_id` is not null and `rewrite_id` = 0
      9.9msmiddleware::checkdate:30receivinghelpdeskask
      Metadata
      Bindings
      • 0. 83001
      • 1. 0
      Backtrace
      • 19. middleware::checkdate:30
      • 20. /vendor/laravel/framework/src/Illuminate/Pipeline/Pipeline.php:167
      • 21. /vendor/laravel/jetstream/src/Http/Middleware/ShareInertiaData.php:61
      • 22. /vendor/laravel/framework/src/Illuminate/Pipeline/Pipeline.php:167
      • 23. /vendor/laravel/framework/src/Illuminate/Routing/Middleware/SubstituteBindings.php:50
    • select * from `nova_menu_menus` where `slug` = 'header' limit 1
      310μs/vendor/outl1ne/nova-menu-builder/src/helpers.php:32receivinghelpdeskask
      Metadata
      Bindings
      • 0. header
      Backtrace
      • 15. /vendor/outl1ne/nova-menu-builder/src/helpers.php:32
      • 17. /vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 18. /vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:45
      • 19. /vendor/laravel/framework/src/Illuminate/Routing/Route.php:261
      • 20. /vendor/laravel/framework/src/Illuminate/Routing/Route.php:205
    • select * from `nova_menu_menu_items` where `nova_menu_menu_items`.`menu_id` = 1 and `nova_menu_menu_items`.`menu_id` is not null and `parent_id` is null order by `parent_id` asc, `order` asc, `name` asc
      280μs/vendor/outl1ne/nova-menu-builder/src/Models/Menu.php:35receivinghelpdeskask
      Metadata
      Bindings
      • 0. 1
      Backtrace
      • 19. /vendor/outl1ne/nova-menu-builder/src/Models/Menu.php:35
      • 20. /vendor/outl1ne/nova-menu-builder/src/helpers.php:33
      • 22. /vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 23. /vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:45
      • 24. /vendor/laravel/framework/src/Illuminate/Routing/Route.php:261
    • select * from `nova_menu_menu_items` where `nova_menu_menu_items`.`parent_id` in (1) order by `order` asc
      200μs/vendor/outl1ne/nova-menu-builder/src/Models/Menu.php:35receivinghelpdeskask
      Metadata
      Backtrace
      • 24. /vendor/outl1ne/nova-menu-builder/src/Models/Menu.php:35
      • 25. /vendor/outl1ne/nova-menu-builder/src/helpers.php:33
      • 27. /vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 28. /vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:45
      • 29. /vendor/laravel/framework/src/Illuminate/Routing/Route.php:261
    • select `id`, `post_title`, `slug` from `posts` where `status` = 'publish' and `posts`.`deleted_at` is null order by RAND() limit 10
      2.18s/app/View/Composers/SidebarView.php:22receivinghelpdeskask
      Metadata
      Bindings
      • 0. publish
      Backtrace
      • 14. /app/View/Composers/SidebarView.php:22
      • 15. /app/View/Composers/SidebarView.php:12
      • 16. /vendor/laravel/framework/src/Illuminate/View/Concerns/ManagesEvents.php:124
      • 17. /vendor/laravel/framework/src/Illuminate/View/Concerns/ManagesEvents.php:162
      • 20. /vendor/laravel/framework/src/Illuminate/View/Concerns/ManagesEvents.php:177
    • select * from `fake_users` where `fake_users`.`id` = 9372 limit 1
      710μsview::2dd102cf0462e89a4d4d8bc77355d767652bf9aa:15receivinghelpdeskask
      Metadata
      Bindings
      • 0. 9372
      Backtrace
      • 21. view::2dd102cf0462e89a4d4d8bc77355d767652bf9aa:15
      • 23. /vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:108
      • 24. /vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 25. /vendor/livewire/livewire/src/ComponentConcerns/RendersLivewireComponents.php:69
      • 26. /vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:61
    App\Models\FakeUser
    1
    Outl1ne\MenuBuilder\Models\MenuItem
    1
    Outl1ne\MenuBuilder\Models\Menu
    1
    App\Models\JsonPostContent
    1
    App\Models\Post
    11
        _token
        zuCMqXhK63KkcXds0K0N2tkeEGbBmYfKcLBJxAgY
        _previous
        array:1 [ "url" => "https://receivinghelpdesk.com/ask/quaternary-protein-structure" ]
        _flash
        array:2 [ "old" => [] "new" => [] ]
        PHPDEBUGBAR_STACK_DATA
        []
        path_info
        /quaternary-protein-structure
        status_code
        200
        
        status_text
        OK
        format
        html
        content_type
        text/html; charset=UTF-8
        request_query
        []
        
        request_request
        []
        
        request_headers
        0 of 0
        array:25 [ "cookie" => array:1 [ 0 => "XSRF-TOKEN=eyJpdiI6InFUSExvVXBGZWF6dUErTmtwUWwzcWc9PSIsInZhbHVlIjoiZk9IZU5NQkVWTitCd29jUk9MVythRzZ3S2xicmN2anhSN0VqNEM0WkZGL3gxTUFuUUcwTk9icFYxdTgvNTBwUERQV1VSQlF5ZjZpNEtrWVhLZzc1L0oyYWdMWUpMSDVZa1d4NFRMenlpTFNsbURuTGRXVTlHQ0tWdk83dGU4Y2YiLCJtYWMiOiIzZTFmNWNjYjc0MzgwNDVjMzY4OWYzMGQyNmQ4OTkxYWFjMWVkZTEzMjljYTY1NjQ3MGQ4ZmY3YjYxMGYxNmE4IiwidGFnIjoiIn0%3D; askhelpdesk_session=eyJpdiI6IlNzdCtEZTQrcHU1NWhVQWYxTkpZd1E9PSIsInZhbHVlIjoiSTNUeTBjeXJJVUp0aUtDRlhjMVY0cVNwQlVmY1VKTVBxeUZHdE8rOGJTUzY2NUFPWlo4VExBeEh6eSs2YXJJOVlNT2hKWE1ja1IvaExnalBTUlR2TVdsWjg0QTZMNGY0djdhTVFYNTh6WGJ1MEgvb3BaaFNCOWtUQXpxUWVyRGIiLCJtYWMiOiJkZTY4NWI5MjYzNzZjNmIxZWJjNTIxNDY0YzdkMDBhYjRmYjg4NDQyMzFhYzk4Y2VkNDY2MDkwYTU0ZDVlMWNmIiwidGFnIjoiIn0%3D; _pk_id.64.7c30=f8c5e313fd38b596.1750329406.; _pk_ses.64.7c30=1XSRF-TOKEN=eyJpdiI6InFUSExvVXBGZWF6dUErTmtwUWwzcWc9PSIsInZhbHVlIjoiZk9IZU5NQkVWTitCd29jUk9MVythRzZ3S2xicmN2anhSN0VqNEM0WkZGL3gxTUFuUUcwTk9icFYxdTgvNTBwUERQV1VSQ" ] "cf-ipcountry" => array:1 [ 0 => "US" ] "cf-connecting-ip" => array:1 [ 0 => "216.73.216.239" ] "cdn-loop" => array:1 [ 0 => "cloudflare; loops=1" ] "sec-fetch-mode" => array:1 [ 0 => "navigate" ] "sec-fetch-site" => array:1 [ 0 => "none" ] "accept" => array:1 [ 0 => "text/html,application/xhtml+xml,application/xml;q=0.9,image/avif,image/webp,image/apng,*/*;q=0.8,application/signed-exchange;v=b3;q=0.7" ] "user-agent" => array:1 [ 0 => "Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)" ] "upgrade-insecure-requests" => array:1 [ 0 => "1" ] "sec-ch-ua-platform" => array:1 [ 0 => ""Windows"" ] "sec-ch-ua-mobile" => array:1 [ 0 => "?0" ] "sec-ch-ua" => array:1 [ 0 => ""Chromium";v="130", "HeadlessChrome";v="130", "Not?A_Brand";v="99"" ] "cache-control" => array:1 [ 0 => "no-cache" ] "pragma" => array:1 [ 0 => "no-cache" ] "accept-encoding" => array:1 [ 0 => "gzip, br" ] "cf-ray" => array:1 [ 0 => "9522632d4c5af4ae-ORD" ] "priority" => array:1 [ 0 => "u=0, i" ] "sec-fetch-dest" => array:1 [ 0 => "document" ] "sec-fetch-user" => array:1 [ 0 => "?1" ] "cf-visitor" => array:1 [ 0 => "{"scheme":"https"}" ] "connection" => array:1 [ 0 => "close" ] "x-forwarded-proto" => array:1 [ 0 => "https" ] "x-forwarded-for" => array:1 [ 0 => "216.73.216.239, 172.69.17.181" ] "x-server-addr" => array:1 [ 0 => "154.12.239.204" ] "host" => array:1 [ 0 => "receivinghelpdesk.com" ] ]
        request_server
        0 of 0
        array:56 [ "USER" => "runcloud" "HOME" => "/home/runcloud" "SCRIPT_NAME" => "/ask/index.php" "REQUEST_URI" => "/ask/quaternary-protein-structure" "QUERY_STRING" => "" "REQUEST_METHOD" => "GET" "SERVER_PROTOCOL" => "HTTP/1.0" "GATEWAY_INTERFACE" => "CGI/1.1" "REDIRECT_URL" => "/ask/quaternary-protein-structure" "REMOTE_PORT" => "50700" "SCRIPT_FILENAME" => "/home/runcloud/webapps/ReceivingHelpDesk/ask/index.php" "SERVER_ADMIN" => "you@example.com" "CONTEXT_DOCUMENT_ROOT" => "/home/runcloud/webapps/ReceivingHelpDesk/" "CONTEXT_PREFIX" => "" "REQUEST_SCHEME" => "http" "DOCUMENT_ROOT" => "/home/runcloud/webapps/ReceivingHelpDesk/" "REMOTE_ADDR" => "172.69.17.181" "SERVER_PORT" => "80" "SERVER_ADDR" => "127.0.0.1" "SERVER_NAME" => "receivinghelpdesk.com" "SERVER_SOFTWARE" => "Apache/2.4.63 (Unix) OpenSSL/1.1.1f" "SERVER_SIGNATURE" => "" "LD_LIBRARY_PATH" => "/RunCloud/Packages/apache2-rc/lib" "PATH" => "/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin" "HTTP_COOKIE" => "XSRF-TOKEN=eyJpdiI6InFUSExvVXBGZWF6dUErTmtwUWwzcWc9PSIsInZhbHVlIjoiZk9IZU5NQkVWTitCd29jUk9MVythRzZ3S2xicmN2anhSN0VqNEM0WkZGL3gxTUFuUUcwTk9icFYxdTgvNTBwUERQV1VSQlF5ZjZpNEtrWVhLZzc1L0oyYWdMWUpMSDVZa1d4NFRMenlpTFNsbURuTGRXVTlHQ0tWdk83dGU4Y2YiLCJtYWMiOiIzZTFmNWNjYjc0MzgwNDVjMzY4OWYzMGQyNmQ4OTkxYWFjMWVkZTEzMjljYTY1NjQ3MGQ4ZmY3YjYxMGYxNmE4IiwidGFnIjoiIn0%3D; askhelpdesk_session=eyJpdiI6IlNzdCtEZTQrcHU1NWhVQWYxTkpZd1E9PSIsInZhbHVlIjoiSTNUeTBjeXJJVUp0aUtDRlhjMVY0cVNwQlVmY1VKTVBxeUZHdE8rOGJTUzY2NUFPWlo4VExBeEh6eSs2YXJJOVlNT2hKWE1ja1IvaExnalBTUlR2TVdsWjg0QTZMNGY0djdhTVFYNTh6WGJ1MEgvb3BaaFNCOWtUQXpxUWVyRGIiLCJtYWMiOiJkZTY4NWI5MjYzNzZjNmIxZWJjNTIxNDY0YzdkMDBhYjRmYjg4NDQyMzFhYzk4Y2VkNDY2MDkwYTU0ZDVlMWNmIiwidGFnIjoiIn0%3D; _pk_id.64.7c30=f8c5e313fd38b596.1750329406.; _pk_ses.64.7c30=1XSRF-TOKEN=eyJpdiI6InFUSExvVXBGZWF6dUErTmtwUWwzcWc9PSIsInZhbHVlIjoiZk9IZU5NQkVWTitCd29jUk9MVythRzZ3S2xicmN2anhSN0VqNEM0WkZGL3gxTUFuUUcwTk9icFYxdTgvNTBwUERQV1VSQ" "HTTP_CF_IPCOUNTRY" => "US" "HTTP_CF_CONNECTING_IP" => "216.73.216.239" "HTTP_CDN_LOOP" => "cloudflare; loops=1" "HTTP_SEC_FETCH_MODE" => "navigate" "HTTP_SEC_FETCH_SITE" => "none" "HTTP_ACCEPT" => "text/html,application/xhtml+xml,application/xml;q=0.9,image/avif,image/webp,image/apng,*/*;q=0.8,application/signed-exchange;v=b3;q=0.7" "HTTP_USER_AGENT" => "Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)" "HTTP_UPGRADE_INSECURE_REQUESTS" => "1" "HTTP_SEC_CH_UA_PLATFORM" => ""Windows"" "HTTP_SEC_CH_UA_MOBILE" => "?0" "HTTP_SEC_CH_UA" => ""Chromium";v="130", "HeadlessChrome";v="130", "Not?A_Brand";v="99"" "HTTP_CACHE_CONTROL" => "no-cache" "HTTP_PRAGMA" => "no-cache" "HTTP_ACCEPT_ENCODING" => "gzip, br" "HTTP_CF_RAY" => "9522632d4c5af4ae-ORD" "HTTP_PRIORITY" => "u=0, i" "HTTP_SEC_FETCH_DEST" => "document" "HTTP_SEC_FETCH_USER" => "?1" "HTTP_CF_VISITOR" => "{"scheme":"https"}" "HTTP_CONNECTION" => "close" "HTTP_X_FORWARDED_PROTO" => "https" "HTTP_X_FORWARDED_FOR" => "216.73.216.239, 172.69.17.181" "HTTP_X_SERVER_ADDR" => "154.12.239.204" "HTTP_HOST" => "receivinghelpdesk.com" "HTTPS" => "on" "REDIRECT_STATUS" => "200" "REDIRECT_HTTPS" => "on" "FCGI_ROLE" => "RESPONDER" "PHP_SELF" => "/ask/index.php" "REQUEST_TIME_FLOAT" => 1750329407.577 "REQUEST_TIME" => 1750329407 ]
        request_cookies
        0 of 0
        array:4 [ "XSRF-TOKEN" => "zuCMqXhK63KkcXds0K0N2tkeEGbBmYfKcLBJxAgY" "askhelpdesk_session" => "F3JzMtsGRIZORPozRMD5ZxcOx338kS08T2GcThjU" "_pk_id_64_7c30" => null "_pk_ses_64_7c30" => null ]
        response_headers
        0 of 0
        array:7 [ "content-type" => array:1 [ 0 => "text/html; charset=UTF-8" ] "cache-control" => array:1 [ 0 => "private, must-revalidate" ] "date" => array:1 [ 0 => "Thu, 19 Jun 2025 10:36:47 GMT" ] "pragma" => array:1 [ 0 => "no-cache" ] "expires" => array:1 [ 0 => -1 ] "set-cookie" => array:2 [ 0 => "XSRF-TOKEN=eyJpdiI6Ik9hWEdXMnRMcjY2QldLalFtVi9KU1E9PSIsInZhbHVlIjoicnhKbC9SN09MeStiZjQ3bEZSN1dBUjh0aForREdOakM5VWN6VzZXaFJxcUpwcnUyWUYzT3N1UjRsTXlkbzVOZDlYUWE2eTFTYTlOWnpXWXE0OFpxWEV6d21jdlZMK1BrZU9tOWRQaUYrRENNd2EwbEVjK2dSeG43OHJPRWlCOSsiLCJtYWMiOiI2YmNjMmMzMzRmMmZiNzk1MDhiOGZkNTdiZDlhMDY4YTQ3YjdlN2NhOGM1Yjk1OTJjMWMxNzU0OTUwMzg5ZTAzIiwidGFnIjoiIn0%3D; expires=Thu, 19-Jun-2025 12:36:49 GMT; Max-Age=7200; path=/; samesite=laxXSRF-TOKEN=eyJpdiI6Ik9hWEdXMnRMcjY2QldLalFtVi9KU1E9PSIsInZhbHVlIjoicnhKbC9SN09MeStiZjQ3bEZSN1dBUjh0aForREdOakM5VWN6VzZXaFJxcUpwcnUyWUYzT3N1UjRsTXlkbzVOZDlYUWE2e" 1 => "askhelpdesk_session=eyJpdiI6InUrbFBFOUkrNWl2OG9PclYwVHlYWlE9PSIsInZhbHVlIjoiU2ppRzlzUlNobjMwWFNHeS94ZmZhSmZnYSsrVk42Tys2Q09qVWFESjFUbVdqZVZzeUJCeFgyT0IyQVdXS3FnNG1Ydk8rbGlzZHVwQUxOYWJFM09RRGlzbUFmaXl5RzdMZEJGdVhjWDdzYlZTUm9mWHk2NTluTTdQTXpmampFalEiLCJtYWMiOiJmNzY0MGNjMzZmYjIwYmY4YTZmMWNmZjVhNjdhOGYwNzgyYjAyZmFlYWMwZGY1ZTgzOTBkYzU4Y2YyOGJlZDcwIiwidGFnIjoiIn0%3D; expires=Thu, 19-Jun-2025 12:36:49 GMT; Max-Age=7200; path=/; httponly; samesite=laxaskhelpdesk_session=eyJpdiI6InUrbFBFOUkrNWl2OG9PclYwVHlYWlE9PSIsInZhbHVlIjoiU2ppRzlzUlNobjMwWFNHeS94ZmZhSmZnYSsrVk42Tys2Q09qVWFESjFUbVdqZVZzeUJCeFgyT0IyQVdXS3Fn" ] "Set-Cookie" => array:2 [ 0 => "XSRF-TOKEN=eyJpdiI6Ik9hWEdXMnRMcjY2QldLalFtVi9KU1E9PSIsInZhbHVlIjoicnhKbC9SN09MeStiZjQ3bEZSN1dBUjh0aForREdOakM5VWN6VzZXaFJxcUpwcnUyWUYzT3N1UjRsTXlkbzVOZDlYUWE2eTFTYTlOWnpXWXE0OFpxWEV6d21jdlZMK1BrZU9tOWRQaUYrRENNd2EwbEVjK2dSeG43OHJPRWlCOSsiLCJtYWMiOiI2YmNjMmMzMzRmMmZiNzk1MDhiOGZkNTdiZDlhMDY4YTQ3YjdlN2NhOGM1Yjk1OTJjMWMxNzU0OTUwMzg5ZTAzIiwidGFnIjoiIn0%3D; expires=Thu, 19-Jun-2025 12:36:49 GMT; path=/XSRF-TOKEN=eyJpdiI6Ik9hWEdXMnRMcjY2QldLalFtVi9KU1E9PSIsInZhbHVlIjoicnhKbC9SN09MeStiZjQ3bEZSN1dBUjh0aForREdOakM5VWN6VzZXaFJxcUpwcnUyWUYzT3N1UjRsTXlkbzVOZDlYUWE2e" 1 => "askhelpdesk_session=eyJpdiI6InUrbFBFOUkrNWl2OG9PclYwVHlYWlE9PSIsInZhbHVlIjoiU2ppRzlzUlNobjMwWFNHeS94ZmZhSmZnYSsrVk42Tys2Q09qVWFESjFUbVdqZVZzeUJCeFgyT0IyQVdXS3FnNG1Ydk8rbGlzZHVwQUxOYWJFM09RRGlzbUFmaXl5RzdMZEJGdVhjWDdzYlZTUm9mWHk2NTluTTdQTXpmampFalEiLCJtYWMiOiJmNzY0MGNjMzZmYjIwYmY4YTZmMWNmZjVhNjdhOGYwNzgyYjAyZmFlYWMwZGY1ZTgzOTBkYzU4Y2YyOGJlZDcwIiwidGFnIjoiIn0%3D; expires=Thu, 19-Jun-2025 12:36:49 GMT; path=/; httponlyaskhelpdesk_session=eyJpdiI6InUrbFBFOUkrNWl2OG9PclYwVHlYWlE9PSIsInZhbHVlIjoiU2ppRzlzUlNobjMwWFNHeS94ZmZhSmZnYSsrVk42Tys2Q09qVWFESjFUbVdqZVZzeUJCeFgyT0IyQVdXS3Fn" ] ]
        session_attributes
        0 of 0
        array:4 [ "_token" => "zuCMqXhK63KkcXds0K0N2tkeEGbBmYfKcLBJxAgY" "_previous" => array:1 [ "url" => "https://receivinghelpdesk.com/ask/quaternary-protein-structure" ] "_flash" => array:2 [ "old" => [] "new" => [] ] "PHPDEBUGBAR_STACK_DATA" => [] ]