How is RF value calculated in HPLC? The Rf value is defined as the ratio of the distance moved by the solute (i.e. the dye or pigment under test) and the distance moved by the the solvent (known as the Solvent front) along the paper, where both distances are measured from the common Origin or Application Baseline, that is the point where the sample is.
How RRF calculated in HPLC analysis?
How RRF calculated in HPLC analysis? In high pressure liquid chromatography ( HPLC ), the compound is injected through a column of different sized beads.The amount of time it takes for the compound to pass through the column is the retention time (RT). Join ResearchGate to ask questions, get input, and advance your work.
How to calculate Rf values?
How to Calculate RF Values. The retention factor (rf) of a particular material is the ratio of the distance the spot moved above the origin to the distance the solvent front moved above the origin. Retention factors range between zero and one.
What are the factors affecting the Rf value in TLC?
The differences in the solvent polarity can be affecting the Rf value. Solvent impact on retention factors: Since the solvent carries the analyte up the TLC plate, the solvent used also will affect the value of the component retention factor.
Can relative response factor be used to calculate product determined in HPLC?
Relative response factor determined on a particular detector cannot be used in the calculation of product determined on another detector because different detectors show the difference in the response of the same compound. Solutions of at least three different concentrations of standard and impurity are prepared and injected in HPLC.
Why does the Rf value depend on the relative affinity of analytes for the absorbent?
How does solvent affect Rf?
What is the retention factor of a chemical?
Does the solvent mixture affect the Rf value?
Does sample application affect retention factor?
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What is Rf value in HPLC?
Retardation or retention factor (Rf) value is the ratio of distance traveled by the analyte to that of the solvent front on a chromatogram. The chromatographic techniques in which the analytes are added to the stationary phases show a difference in the movement of analytes with mobile solvents (phases).Oct 18, 2021
How do you calculate the Rf value in chromatography?
Measure the distance between the base pencil line and the pencil line you used to mark the solvent front. This is the distance travelled by the solvent. Divide the distance travelled by the component by the distance travelled by the solvent. This gives you your Rf value.
What is an Rf value?
The ratio of the distance the compound travels to the distance the solvent travels is called the Rf value. The symbol Rf stands for "retardation factor" or "ratio-to-front".
What is Rf formula?
The following expression can be used to calculate Rf values: Rf = Distance travelled by the substance from reference line (cm)/Distance travelled by the solvent front from reference line (cm)
Why do RF values differ? - FindAnyAnswer.com
Click to see full answer Also know, what would the same or similar RF values indicate? “Relative Rf” means that the values are reported relative to a standard, or it means that you compare the Rf values of compounds run on the same plate at the same time. If two substances have the same Rf value, they are likely (but not necessarily) the same compound.
Factors Affecting RF Values in Thin Layer Chromatography
Retention Factors.The amount that each component of a mixture travels can be quantified using retention factors (Rf). The retention factor of a particular material is the ratio of the distance the spot moved above the origin to the distance the solvent front moved above the origin.
Chrominfo: Factors affecting Rf value in paper chromatography
Paper chromatography is a chromatographic method of separation in which the flow of solvent separates the substance by capillary action on the specially designed paper (cellulose filter paper) that acts as a stationary phase.
Factors which influence paper chromatographic RF values : Introductory ...
As an introduction to the discussion on the reproducibility of spot positions in paper chromatography, attention is drawn to the role of paper, the volume and composition of solvents (eluents and stationary phases), the atmosphere of the tank (the problems of conditioning and temperature control), development procedures, and additional components of the sample.
Why does the Rf value depend on the relative affinity of analytes for the absorbent?
Because the Rf value depends on the relative affinity of analytes for the absorbent, modifying the absorbent can change the retention factor significantly. Also, the thickness of the absorbents layer and uniformity may vary from plate to plate. These factors also cause to change retention factor values for components.
How does solvent affect Rf?
The differences in the solvent polarity can be affecting the Rf value. Solvent impact on retention factors: Since the solvent carries the analyte up the TLC plate, the solvent used also will affect the value of the component retention factor. A solvent which has a strong interaction for a specific analyte will more easily remove and carry ...
What is the retention factor of a chemical?
During the thin layer chromatography, the retention factor of a chemical is the calculation of how distant the plate is taken in response to the movement of the solvent. Because the movement of chemical relies on how far the solvent travel is, we determine the retention factor values relative to the degree of solvent travels. The Rf for a particular chemical is the vertical distance traveled by a chemical from the place where it was originally spotted on the TLC plate, divided by the length traveled.#N#Retention factor or RF value is the difference in the movement rate of analytes in chromatography is due to various factors, some of them are mentioned here.
Does the solvent mixture affect the Rf value?
The solvent mixture also has effects on the Rf value which is depending on the proportion of each solvent. There may be slight changes in the temperature of the solvent and the plate can affect Rf value in chromatography, although the working temperature needs to monitor.
Does sample application affect retention factor?
The technique of sample applying can also change the retention factor, the more amount of the sample may result in a large and diffuse band of component moving up the plate, makes it obscure to calculate the distance correctly. In some cases, several other factors can affect the retention factor in chromatography.
Most recent answer
Ok noted. Hopefully my compound doesn't affect the polarity since it forms many spots. Thanks Moacir Brito
All Answers (12)
Hello Lee Roy Toh! Do consider the role of solvent also to solve your problem.
Similar questions and discussions
Is there any TCL manager and TLC Rf calculator App, which can calculate Rf values from TLC images ?
Why does the Rf value depend on the relative affinity of analytes for the absorbent?
Because the Rf value depends on the relative affinity of analytes for the absorbent, modifying the absorbent can change the retention factor significantly. Also, the thickness of the absorbents layer and uniformity may vary from plate to plate. These factors also cause to change retention factor values for components.
How does solvent affect Rf?
The differences in the solvent polarity can be affecting the Rf value. Solvent impact on retention factors: Since the solvent carries the analyte up the TLC plate, the solvent used also will affect the value of the component retention factor. A solvent which has a strong interaction for a specific analyte will more easily remove and carry ...
What is the retention factor of a chemical?
During the thin layer chromatography, the retention factor of a chemical is the calculation of how distant the plate is taken in response to the movement of the solvent. Because the movement of chemical relies on how far the solvent travel is, we determine the retention factor values relative to the degree of solvent travels. The Rf for a particular chemical is the vertical distance traveled by a chemical from the place where it was originally spotted on the TLC plate, divided by the length traveled.#N#Retention factor or RF value is the difference in the movement rate of analytes in chromatography is due to various factors, some of them are mentioned here.
Does the solvent mixture affect the Rf value?
The solvent mixture also has effects on the Rf value which is depending on the proportion of each solvent. There may be slight changes in the temperature of the solvent and the plate can affect Rf value in chromatography, although the working temperature needs to monitor.
Does sample application affect retention factor?
The technique of sample applying can also change the retention factor, the more amount of the sample may result in a large and diffuse band of component moving up the plate, makes it obscure to calculate the distance correctly. In some cases, several other factors can affect the retention factor in chromatography.
