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how do you calculate log cfu ml

by Gabe Aufderhar Published 3 years ago Updated 2 years ago

Calculating logs To work out the result from a lab report, you need to multiply the result before the 10 log by the log value. Like this: Result x 10 log = cfu count/ml or g

If your CFU is 25, your aliquot is 1cc, and your dilution factor is 10-2, the calculation of the log CFU/mL is: Log CFU/mL = Log10(CFU / (dilution factor*aliquot)) = Log10(25/(10-2*1)) = 3.40.Jun 25, 2016

Full Answer

How do you calculate CFU in microbiology?

CFU/mL= (Number of bacterial colonies counted on plate x Dilution Factor) / Volume of culture plate CFU/mL= (150 x 105) / 0.1 = 1.50 x 108 So, total colony forming unit = 1.5 x 108 per mL

Is it necessary to convert CFU/g to log (CFU +1)?

Is it necessary to convert CFU/g to log (CFU +1)? I have read that CFU/g is converted to log CFU normally, especially for generate graph. as Changing the numbers to the logarithm makes it easier to see small numbers and large numbers on the same graph.

What is the difference between CFU/g and CFU/mL?

A specific result (e.g. 100cfu/g) Results like this are either provided in cfu/g or cfu/ml. cfu stands for colony-forming unit. This means that cfu/g is colony-forming unit per gram and cfu/ml is colony-forming unit per millilitre.

How do you calculate colony forming unit per mL of solution?

If you overlay 100μL (which is equivalent to 0.1 mL) this solution onto agar plate and got 36 colonies, the number of colony forming unit per mL is calculated as: = 360/0.1 = 3600. Therefore, number of colony forming unit per mL of concerned solution is 3600.

What is log CFU ml?

The formula is calculated as log (cfu/g) : Number of colony/dilution × volume of sample taken × 1/weight of sample. Since 0.1 ml = 1/10ml. Number of colony/10-4 × 1/10 × 1/weight of sample.

What is the CFU formula?

The CFU/ml can be calculated using the formula: cfu/ml = (no. of colonies x dilution factor) / volume of culture plate. For example, suppose the plate of the 10^6 dilution yielded a count of 130 colonies.

How do you calculate CFU of bacteria?

As far as I know, dilution factor = volume of sample/total volume and CFU = (no colony x dilution factor)/volume plated in mL. For example, if I added 10gr of sample into 90mL sterilized Ringer's solution.

How do you calculate log CFU cm2?

An example of a calculation using data from five carcasses is as follows:Cfu/cm2 = Average cfu/plate x a (volume of original suspension)b (total surface area e.g. 5cm2 x 4 excisions) x (dilution factor)= 4250 cfu/cm2.

How do you calculate log reduction in microbiology?

Log Reduction CalculatorFormula. Log Reduction = log10 (A/B)Initial Quantity.Final Quantity (after treatment for microorganisms)

What does CFU 100 mL mean?

colony forming unitsCFU/100 mL = (# of colonies counted ÷ sample volume filtered in mL) x 100 (CFU = colony forming units). 2.0. Colony Counts Within the Ideal Counting Range. The ideal range of colonies that are countable on a membrane filter depends on the method and/or regulatory guidelines.

What does 105 CFU mL mean?

Asymptomatic bacteriuria denotes significant bacteriuria (> 105 CFU/mL of urine) without clinical symptoms or other abnormal findings. Lower urinary tract infection refers to infection at or below the level of the bladder.

Is CFU mL the same as cells mL?

In my knowledge yes they are same. When you do plate count of bacteria you make serial suspension- dilution in saline buffer or any other diluent, and then you plate a certain volume (100 microliter on a 9cm Petri plate) you spread and incubate and then you count the number of colonies formed.

How do you calculate bacterial growth?

The rate of exponential growth of a bacterial culture is expressed as generation time, also the doubling time of the bacterial population. Generation time (G) is defined as the time (t) per generation (n = number of generations). Hence, G=t/n is the equation from which calculations of generation time (below) derive.

How do you calculate logs?

We can easily calculate that ln 10 = 2.302585093... or 2.303 and log 10 = 1. So, the number has to be 2.303....CALCULATIONS INVOLVING LOGARITHMS.Common LogarithmNatural Logarithmlog x/y = log x - log yln x/y = ln x - ln ylog xy = y log xln xy = y ln xlog = log x1/y = (1/y )log xln = ln x1/y =(1/y)ln x1 more row

What is the value of log10?

Value of Log 1 to 10 for Log Base 10Common Logarithm to a Number (log10 x)Log ValueLog 70.8450Log 80.9030Log 90.9542Log 1016 more rows

What is log10 reduction?

Log Reduction stands for a 10-fold (one decimal) or 90% reduction in numbers of live bacteria. Another way to look at it is: 1-Log Reduction would reduce the number of bacteria 90%. This means, for example, that 100 bacteria would be reduced to 10, or 10 reduced to 1.

What does log cfu/g mean?

A log is a multiple of 10, that’s used for reporting micro. cfu/g means colony-forming unit per gram. It’s basically, the number of colonies counte...

What is ‘D’ and ‘ND’ on my lab report?

The lab has reported that they have either detected the bacteria (reported as ‘D’) or not detected the bacteria (reported as ‘ND’).

What’s a less than result mean?

What this means is, that the lab has not seen any colony-forming units on the petri dish at all. But they can’t say that the result is ‘0’ (zero),...

What’s a greater than result mean?

You’ll get a greater than result, if the results are really high. When this happens and the plate is too full to count. This means, that the lab ca...

What do I need to do if my sample is expected to have a high count?

Ask the lab to dilute your sample so you can get an accurate count.

What size area should I swab if the lab hasn’t specified this?

Any size is OK as long as you record the size of the area that you’ve swabbed so you can interpret the result when you get it back.

Why are logarithms used by the lab when they send the results back to me?

The count on the plate you sent was high, so they’ve shown the result using a log count to reduce the number of zero’s shown in the result.

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