What is BSA used for in microbiology?
Another use for BSA is that it can be used to temporarily isolate substances that are blocking the activity of the enzyme that is needed, thus impeding polymerase chain reaction (PCR). BSA has been widely used as a template to synthesize nanostructures. BSA is also the main constituent of fetal bovine serum, a common cell culture medium.
What is UniProt?
The mission of UniProt is to provide the scientific community with a comprehensive, high-quality and freely accessible resource of protein sequence and functional information. Manually annotated and reviewed.
What is the'basket'in the UniProtKB?
When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. More... The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome.
What is the purpose of using BSA in PCR?
BSA is used because of its ability to increase signal in assays, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry.
What is UniProt used for?
UniProt is the central resource for storing and interconnecting information from large and disparate sources, and the most comprehensive catalog of protein sequence and functional annotation.
What is UniProt KB?
The UniProt Knowledgebase (UniProtKB) is the central hub for the collection of functional information on proteins, with accurate, consistent and rich annotation.
Is swissprot and UniProt same?
Swiss-Prot (created in 1986) is a high quality manually annotated and non-redundant protein sequence database, which brings together experimental results, computed features and scientific conclusions. UniProtKB/Swiss-Prot is now the reviewed section of the UniProt Knowledgebase.
What is UniProt ID?
The proteome identifier (UPID) is the unique identifier assigned to the set of proteins that constitute the proteome. It consists of the characters 'UP' followed by 9 digits, is stable across releases and can therefore be used to cite a UniProt proteome. UniProtKB entries can be linked to one or more UPIDs.
How do I find my UniProt ID?
Select the Retrieve/ID mapping tab of the toolbar and enter or upload a list of identifiers (or gene names) to do one of the following: Retrieve the corresponding UniProt entries to download them or work with them on this website.
What are the features of UniProt?
THE UNIPROT KNOWLEDGEBASE (UNIPROT)High-quality annotation. ... Manual annotation by curators based on literature and sequence analysis. ... Automatic classification and annotation. ... High-quality Automated and Manual Annotation of microbial Proteomes (HAMAP) ... Standardized nomenclature and controlled vocabularies.More items...
Is UniProt a secondary database?
Hybrid databases and families of databases Many data resources have both primary and secondary characteristics. For example, UniProt accepts primary sequences derived from peptide sequencing experiments.
How many proteins are in UniProt?
UniProt release 2020_04 contains over 189 million sequence records (Figure 1), with >292 000 proteomes, the complete set of proteins believed to be expressed by an organism, originating from completely sequenced viral, bacterial, archaeal and eukaryotic genomes available through the UniProtKB Proteomes portal (https:// ...
Who created Swiss-Prot?
SWISS-PROT (1) is an annotated protein sequence database, which was created at the Department of Medical Biochemistry of the University of Geneva and has been a collaborative effort of the Department and the European Molecular Biology Laboratory (EMBL), since 1987.
How do I get my UniProt ID from NCBI?
You can click directly on the 'Download' button in the basket to retrieve the UniProt entries in your basket. To map the UniProt IDs to an external database from the basket, click on the checkboxes to the left and click on 'Map IDs' button in the basket, as shown in Figure 11.
Can you cite UniProt?
https://www.uniprot.org/uniparc/UPI00000002E4. Remarks: A UniProtKB accession number (AC) is a stable identifier and therefore allows unambiguous citation of a UniProtKB entry. This is not the case for the 'Entry name'.
How do I download UniProt sequences?
Retrieving sequences from the websitePerform your favorite query and view the resulting list of entries (e.g. this query retrieves all UniProtKB entries that are part of the human proteome: proteome:UP000005640)Click the Download button in the query result page.More items...•
What is an annotation score?
The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score cannot be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.
Does enterobactin inhibit iron uptake?
Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed: 6234017 ). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed: 6234017 ).
Description
Contains 583 amino acid residues derived from the prototypical bovine serum albumin sequence. Suitable for use as a biochemical, excipient (an inert substance used as a diluent or vehicle for a protein), in culture media and chromatographic applications.
Storage & Handling
Lyophilized BSA although stable at room temperature for 3 weeks, should be stored 2-8°C. Upon reconstitution BSA should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Cite This Product
BSA Bovine Serum Albumin protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP02769)
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
For more info on ALB, check out the ALB Infographic
In this infographic you will see the following information for ALB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways.
What is BSA in biology?
InterPro. Bovine serum albumin ( BSA or "Fraction V") is a serum albumin protein derived from cows. It is often used as a protein concentration standard in lab experiments.
Why is BSA used in immunohistochemistry?
Because BSA is a small, stable, moderately non-reactive protein, it is often used as a blocker in immunohistochemistry. During immunohistochemistry, which is the process that uses antibodies to identify antigens in cells, tissue sections are often incubated with BSA blockers to bind nonspecific binding sites.
What is BSA used for?
In restriction digests, BSA is used to stabilize some enzymes during the digestion of DNA and to prevent adhesion of the enzyme to reaction tubes, pipette tips, and other vessels.