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are there any disadvantages to the wet mount technique

by Prof. Abbie Wyman III Published 3 years ago Updated 2 years ago

Name two disadvantages to a wet-mount: movement of specimen and evaporation of liquid. Your objective is to have sufficient water to fill the space between cover slip and slide.. Disadvantages: This technique takes longer to provide results than the wet mount or hanging drop. Author: ESTRIDGE. 1. 1.

One of the drawbacks of iodine wet mounts is that the cysts are stained poorly with the iodine and are less retractile than unstained cysts in the saline preparation; therefore, they are difficult to visualize and can easily be missed.

Full Answer

What are the advantages and disadvantages of wet mount and hanging drop?

Advantages: Like the wet mount, the hanging drop method preserves cell shape and arrangement. The Vaseline-sealed depression also slows down the drying-out process, so the organisms can be observed for longer periods. Disadvantages: The hanging drop method is also far too risky to use with highly pathogenic organisms.

What is a wet mount?

For this reason, wet mounts are the first kind of mounts that students learn to make. Few artifacts: If there is no chemical and physical processing of the specimens before observation (no fixation), there are little artifacts and the specimens appear in their natural condition.

What are the disadvantages of hanging drop method?

Disadvantages of the hanging drop technique include the possibility of overlooking a few motile cells among countless nonmotile cells, and the observation process can be time consuming. A third method of testing for organism motility is the semisolid motility test.

What are the advantages and disadvantages of wet mounts?

Wet-mount Slides Advantages - This type of slide preparation allows you to view microscopic living things without them drying out. Disadvantages - It takes a bit more time to prepare these slides.

What are the disadvantages of wet mount?

Disadvantages: -Movement of specimens make it difficult to draw/photograph. -Evaporation of liquid. -Focus-some organisms may swim vertically, moving in and out of focus.

What are the advantages and disadvantages of using the hanging drop technique?

Advantages: Like the wet mount, the hanging drop method preserves cell shape and arrangement. The Vaseline-sealed depression also slows down the drying-out process, so the organisms can be observed for longer periods. Disadvantages: The hanging drop method is also far too risky to use with highly pathogenic organisms.

What is the advantage of using a wet mount on a microscope?

Wet mount slide preparation allows the viewing of specimen behavior as they interact with the environment.

What is wet mount technique?

The Wet Mount is a procedure performed in the laboratory to observe motile organisms. It is commonly used to examine material collected from the vaginal wall of a female patient.

What is the major advantage of using living cell preparations hanging drop or wet mount rather than stained preparations?

what is a major advantage of using living cell preparations (hanging-drop or wet mount) rather than stained preparations? With living cell preparations, the motility of the bacteria can be seen, as well as the original shape and size of the bacteria, and binary fission.

What is the advantage of using a wet mount?

What is the advantage of using a wet mount? The motility of a specimen can be viewed under a microscope, The specimen can be viewed as living cells.

What are the advantages of using a hanging drop mount rather than a wet mount?

It does not distort the cell shape and arrangement. Besides, the hanging drop method provides a better view of motility in bacteria than the wet mount method. It is also helpful in the classification of the bacteria, whether they are motile or immotile.

What are the advantages of hanging drop over a wet mount technique?

The wet mount tend to dry out quickly under the heat of the microscope light; it is simpler to perform than the wet mount, but it is useful for short-term observation only. The hanging drop is a more complex technique, but it allows for longer-term obervation and more reliable observation of motility.

Is a wet mount a safer way to view pathogenic organisms?

The wet mount is a safer way to view pathogenic organisms. The specimen can be viewed as living cells. The contrast of a specimen is enhanced with the addition of water to the slide. A wet mount requires the addition of certain dyes and cell fixatives approximately 12 hours before viewing the specimen.

Why wet mounts are not observed using oil immersion lens?

DO NOT USE THE OIL IMMERSION LENS FOR A WET MOUNT. The organisms will be very small, due to the low magnification. Often the highest concentration of organism are seen along the edge of the drop.

What are the differences between wet mount and dry mount?

Wet mounting requires the image to be mounted on a foam board that is often the same size as the poster, or is centered within the poster board using either a glue stick or spray adhesive. Dry mounting is a method of setting the image on a hard backing through the use of a heat-sensitive adhesive material.

What is the difference between a wet mount and a dry mount microscope?

Wet mounting requires the image to be mounted on a foam board that is often the same size as the poster, or is centered within the poster board using either a glue stick or spray adhesive. Dry mounting is a method of setting the image on a hard backing through the use of a heat-sensitive adhesive material.

What are the benefit of preparing a good wet mount?

Why would use a wet mount? To increase the specimens translucency and to make it easier to stain. Using a wet mount slide has the tendency to flatten the specimen making it easier to view.

What specimens are examined using wet mounts?

Protozoan trophozoites, cysts, oocysts, and helminth eggs and larvae may be seen and identified using a wet mount identification technique. To prepare a wet mount, obtain a microscope slide and the stool specimen. Take a small amount of the specimen and place it on a microscope slide.

What is the purpose of the direct wet examination?

The direct wet mount examination of soft-to-watery fecal specimens has a unique advantage that can detect the motile trophozoite stage of the protozoan species.

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